2026
Locations of consecutive G・C base pairs direct genomic nucleosome positioning.
Kato H, Fuse T, Sato S, Kurihara Y, Kagawa W, Urano T, Ohkawa Y, Kurumizaka H, Shimizu M.
Genome Research (in press) DOI: 10.1101/gr.281372.125
Structural basis of nucleosome remodeling by Cockayne syndrome B homologue Komagataella phaffii Rad26.
Fukushima Y, Kinoshita C, Negishi L, Kujirai T, Kobayashi Y, Ogasawara M, Ehara H, Sekine SI, Kagawa W, Kurumizaka H, Takizawa Y.
Nature Communications 17, 4881. DOI: 10.1038/s41467-026-73500-7
2024
Heavy water inhibits DNA double-strand break repairs and disturbs cellular transcription, presumably via quantum-level mechanisms of kinetic isotope effects on hydrolytic enzyme reactions.
Yasuda T, Nakajima N, Ogi T, Yanaka T, Tanaka I, Gotoh T, Kagawa W, Sugasawa K, Tajima K.
PLoS One 19, e0309689. DOI: 10.1371/journal.pone.0309689
2023
Structural Basis of Damaged Nucleotide Recognition by Transcribing RNA Polymerase II in the Nucleosome.
Osumi K, Kujirai T, Ehara H, Ogasawara M, Kinoshita C, Saotome M, Kagawa W, Sekine SI, Takizawa Y, Kurumizaka H.
Journal of Molecular Biology 435, 168130. DOI: 10.1016/j.jmb.2023.168130
Biochemical characterization of the RNA-binding and RNA-DNA strand exchange activities of the human RAD52 protein.
Tsuchiya R, Saotome M, Kinoshita C, Kamoi K, Kagawa W.
Journal of Biochemistry 174, 59-69. DOI: 10.1093/jb/mvad019
The cryo-EM structure of full-length RAD52 protein contains an undecameric ring.
Kinoshita C, Takizawa Y, Saotome M, Ogino S, Kurumizaka H, Kagawa W.
FEBS Open Bio 13, 408-418. DOI: 10.1002/2211-5463.13565
2021
Structural basis for DNA sequence recognition by pioneer factors in nucleosomes.
Kagawa W, Kurumizaka H.
Currrent Opinion in Structural Biology 71, 59-64. DOI: 10.1016/j.sbi.2021.05.011
Human SIRT2 and SIRT3 deacetylases function in DNA homologous recombinational repair.
Yasuda T, Takizawa K, Ui A, Hama M, Kagawa W, Sugasawa K, Tajima K.
Genes to Cells 26, 328-335. DOI: 10.1111/gtc.12842
2020
DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres.
Onaka AT, Su J, Katahira Y, Tang C, Zafar F, Aoki K, Kagawa W, Niki H, Iwasaki H, Nakagawa T.
Communications Biology 3, 202. DOI: 10.1038/s42003-020-0934-0
Improved Methods for Preparing the Telomere Tethering Complex Bqt1-Bqt2 for Structural Studies.
Yuzurihara H, Aizawa Y, Saotome M, Ichikawa Y, Yokoyama H, Chikashige Y, Haraguchi T, Hiraoka Y, Kurumizaka H, Kagawa W.
Protein Journal 39, 174-181. DOI: 10.1007/s10930-020-09887-z
2019
A novel mode of DnaA-DnaA interaction promotes ADP dissociation for reactivation of replication initiation activity.
Sugiyama R, Kasho K, Miyoshi K, Ozaki S, Kagawa W, Kurumizaka H, Katayama T.
Nucleic Acids Research 47, 11209-11224. DOI: 10.1093/nar/gkz795
Structure determination of the nucleosome core particle by selenium SAD phasing.
Saotome M, Horikoshi N, Urano K, Kujirai T, Yuzurihara H, Kurumizaka H, Kagawa W.
Acta Crystallographica Section D: Structural Biology 75, 930-936. DOI: 10.1107/S2059798319012713
2018
Structural Basis of Homology-Directed DNA Repair Mediated by RAD52.
Saotome M, Saito K, Yasuda T, Ohtomo H, Sugiyama S, Nishimura Y, Kurumizaka H, Kagawa W.
iScience 3, 50-62. DOI: 10.1016/j.isci.2018.04.005
Novel function of HATs and HDACs in homologous recombination through acetylation of human RAD52 at double-strand break sites.
Yasuda T, Kagawa W, Ogi T, Kato TA, Suzuki T, Dohmae N, Takizawa K, Nakazawa Y, Genet MD, Saotome M, Hama M, Konishi T, Nakajima NI, Hazawa M, Tomita M, Koike M, Noshiro K, Tomiyama K, Obara C, Gotoh T, Ui A, Fujimori A, Nakayama F, Hanaoka F, Sugasawa K, Okayasu R, Jeggo PA, Tajima K.
PLoS Genetics 14, e1007277. DOI: 10.1371/journal.pgen.1007277
2016
Structure of the human DNA-repair protein RAD52 containing surface mutations.
Saotome M, Saito K, Onodera K, Kurumizaka H, Kagawa W.
Acta Crystallographica Section F: Structural Biology Communications 72, 598-603. DOI: 10.1107/S2053230X1601027X
Crystal structure of the nucleosome containing ultraviolet light-induced cyclobutane pyrimidine dimer.
Horikoshi N, Tachiwana H, Kagawa W, Osakabe A, Matsumoto S, Iwai S, Sugasawa K, Kurumizaka H.
Biochemical and Biophysical Research Communications 471, 117-122. DOI: 10.1016/j.bbrc.2016.01.170
Histone H3.5 forms an unstable nucleosome and accumulates around transcription start sites in human testis.
Urahama T, Harada A, Maehara K, Horikoshi N, Sato K, Sato Y, Shiraishi K, Sugino N, Osakabe A, Tachiwana H, Kagawa W, Kimura H, Ohkawa Y, Kurumizaka H.
Epigenetics & Chromatin 9, 2. DOI: 10.1186/s13072-016-0051-y
2015
Structural basis of pyrimidine-pyrimidone (6-4) photoproduct recognition by UV-DDB in the nucleosome.
Osakabe A, Tachiwana H, Kagawa W, Horikoshi N, Matsumoto S, Hasegawa M, Matsumoto N, Toga T, Yamamoto J, Hanaoka F, Thomä NH, Sugasawa K, Iwai S, Kurumizaka H.
Scientific Reports 5, 16330. DOI: 10.1038/srep16330
Structure of RizA, an L-amino-acid ligase from Bacillus subtilis.
Kagawa W, Arai T, Ishikura S, Kino K, Kurumizaka H.
Acta Crystallographica Section F: Structural Biology Communications 71, 1125-1130. DOI: 10.1107/S2053230X15012698
2014
Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3.
Arimura Y, Shirayama K, Horikoshi N, Fujita R, Taguchi H, Kagawa W, Fukagawa T, Almouzni G, Kurumizaka H.
Scientific Reports 4, 7115. DOI: 10.1038/srep07115
Molecular mechanisms of homologous recombination promoted by budding yeast Rad52.
Arai N, Kagawa W.
Seikagaku 86, 693-697.
Functional analyses of the C-terminal half of the Saccharomyces cerevisiae Rad52 protein.
Kagawa W, Arai N, Ichikawa Y, Saito K, Sugiyama S, Saotome M, Shibata T, Kurumizaka H.
Nucleic Acids Research 42, 941-951. DOI: 10.1093/nar/gkt986
2013
Structural polymorphism in the L1 loop regions of human H2A.Z.1 and H2A.Z.2.
Horikoshi N, Sato K, Shimada K, Arimura Y, Osakabe A, Tachiwana H, Hayashi-Takanaka Y, Iwasaki W, Kagawa W, Harata M, Kimura H, Kurumizaka H.
Acta Crystallographica Section D: Biological Crystallography 69, 2431-2439. DOI: 10.1107/S090744491302252X
Contribution of histone N-terminal tails to the structure and stability of nucleosomes.
Iwasaki W, Miya Y, Horikoshi N, Osakabe A, Taguchi H, Tachiwana H, Shibata T, Kagawa W, Kurumizaka H.
FEBS Open Bio 3, 363-369. DOI: 10.1016/j.fob.2013.08.007
Crystallization and preliminary X-ray diffraction analysis of the secreted protein Athe_0614 from Caldicellulosiruptor bescii.
Yokoyama H, Yamashita T, Horikoshi N, Kurumizaka H, Kagawa W.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications 69, 438-440. DOI: 10.1107/S174430911300554X
Purification and characterization of the fission yeast telomere clustering factors, Bqt1 and Bqt2.
Ichikawa Y, Kagawa W, Saito K, Chikashige Y, Haraguchi T, Hiraoka Y, Kurumizaka H.
Protein Expression and Purification 88, 207-213. DOI: 10.1016/j.pep.2013.01.006
Current progress on structural studies of nucleosomes containing histone H3 variants.
Kurumizaka H, Horikoshi N, Tachiwana H, Kagawa W.
Current Opinion in Structural Biology 23, 109-115. DOI: 10.1016/j.sbi.2012.10.009
2012
Histone chaperone activity of Fanconi anemia proteins, FANCD2 and FANCI, is required for DNA crosslink repair.
Sato K, Ishiai M, Toda K, Furukoshi S, Osakabe A, Tachiwana H, Takizawa Y, Kagawa W, Kitao H, Dohmae N, Obuse C, Kimura H, Takata M, Kurumizaka H.
EMBO Journal 31, 3524-3536. DOI: 10.1038/emboj.2012.197
RAD51 plays a crucial role in halting cell death program induced by ionizing radiation in bovine oocytes.
Kujjo LL, Ronningen R, Ross P, Pereira RJ, Rodriguez R, Beyhan Z, Goissis MD, Baumann T, Kagawa W, Camsari C, Smith GW, Kurumizaka H, Yokoyama S, Cibelli JB, Perez GI.
Biology of Reproduction 86, 76. DOI: 10.1095/biolreprod.111.092064
Comparison between the CENP-A and histone H3 structures in nucleosomes.
Tachiwana H, Kagawa W, Kurumizaka H.
Nucleus 3, 6-11. DOI: 10.4161/nucl.18372
2011
Structural basis for the DNA-binding activity of the bacterial β-propeller protein YncE.
Kagawa W, Sagawa T, Niki H, Kurumizaka H.
Acta Crystallographica Section D: Biological Crystallography 67, 1045-1053. DOI: 10.1107/S0907444911045033
Comprehensive structural analysis of mutant nucleosomes containing lysine to glutamine (KQ) substitutions in the H3 and H4 histone-fold domains.
Iwasaki W, Tachiwana H, Kawaguchi K, Shibata T, Kagawa W, Kurumizaka H.
Biochemistry 50,7822-7832. DOI: 10.1021/bi201021h
Crystal structure of the human centromeric nucleosome containing CENP-A.
Tachiwana H, Kagawa W, Shiga T, Osakabe A, Miya Y, Saito K, Hayashi-Takanaka Y, Oda T, Sato M, Park SY, Kimura H, Kurumizaka H.
Nature 476, 232-235. DOI: 10.1038/nature10258
Structures of human nucleosomes containing major histone H3 variants.
Tachiwana H, Osakabe A, Shiga T, Miya Y, Kimura H, Kagawa W, Kurumizaka H.
Acta Crystallographica Section D: Biological Crystallography 67, 578-583. DOI: 10.1107/S0907444911014818
Vital roles of the second DNA-binding site of Rad52 protein in yeast homologous recombination.
Arai N, Kagawa W, Saito K, Shingu Y, Mikawa T, Kurumizaka H, Shibata T.
Journal of Biological Chemistry 286, 17607-17617. DOI: 10.1074/jbc.M110.216739
Structural and biochemical analyses of the human PAD4 variant encoded by a functional haplotype gene.
Horikoshi N, Tachiwana H, Saito K, Osakabe A, Sato M, Yamada M, Akashi S, Nishimura Y, Kagawa W, Kurumizaka H.
Acta Crystallographica Section D: Biological Crystallography 67, 112-118. DOI: 10.1107/S0907444910051711
2010
Structural basis of instability of the nucleosome containing a testis-specific histone variant, human H3T.
Tachiwana H, Kagawa W, Osakabe A, Kawaguchi K, Shiga T, Hayashi-Takanaka Y, Kimura H, Kurumizaka H.
Proceedings of the National Academy of Sciences USA 107, 10454-10459. DOI: 10.1073/pnas.1003064107
The putative nuclear localization signal of the human RAD52 protein is a potential sumoylation site.
Saito K, Kagawa W, Suzuki T, Suzuki H, Yokoyama S, Saitoh H, Tashiro S, Dohmae N, Kurumizaka H.
Journal of Biochemistry 147, 833-842. DOI: 10.1093/jb/mvq020
Enhancing survival of mouse oocytes following chemotherapy or aging by targeting Bax and Rad51.
Kujjo LL, Laine T, Pereira RJ, Kagawa W, Kurumizaka H, Yokoyama S, Perez GI.
PLoS One 5, e9204. DOI: 10.1371/journal.pone.0009204
Dynamics in the transmission of genetic information: from meiosis to postmeiotic events.
Kagawa W, Kurumizaka H.
FEBS Journal 277, 564. DOI: 10.1111/j.1742-4658.2009.07500.x
From meiosis to postmeiotic events: uncovering the molecular roles of the meiosis-specific recombinase Dmc1.
Kagawa W, Kurumizaka H.
FEBS Journal 277, 590-598. DOI: 10.1111/j.1742-4658.2009.07503.x
2008
Biochemical analysis of the N-terminal domain of human RAD54B.
Sarai N, Kagawa W, Fujikawa N, Saito K, Hikiba J, Tanaka K, Miyagawa K, Kurumizaka H, Yokoyama S.
Nucleic Acids Research 36, 5441-5450. DOI: 10.1093/nar/gkn516
Identification of a second DNA binding site in the human Rad52 protein.
Kagawa W, Kagawa A, Saito K, Ikawa S, Shibata T, Kurumizaka H, Yokoyama S.
Journal of Biological Chemistry 283, 24264-24273. DOI: 10.1074/jbc.M802204200
Structural and functional analyses of the DMC1-M200V polymorphism found in the human population.
Hikiba J, Hirota K, Kagawa W, Ikawa S, Kinebuchi T, Sakane I, Takizawa Y, Yokoyama S, Mandon-Pépin B, Nicolas A, Shibata T, Ohta K, Kurumizaka H.
Nucleic Acids Research 36, 4181-4190. DOI: 10.1093/nar/gkn362
A common mechanism for the ATP-DnaA-dependent formation of open complexes at the replication origin.
Ozaki S, Kawakami H, Nakamura K, Fujikawa N, Kagawa W, Park SY, Yokoyama S, Kurumizaka H, Katayama T.
Journal of Biological Chemistry 283, 8351-8362. DOI: 10.1074/jbc.M708684200
2007
The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP DnaA-specific initiation complexes.
Keyamura K, Fujikawa N, Ishida T, Ozaki S, Su’etsugu M, Fujimitsu K, Kagawa W, Yokoyama S, Kurumizaka H, Katayama T.
Genes and Development 21, 2083-2099. DOI:10.1101/gad.1561207
Genetic variance modifies apoptosis susceptibility in mature oocytes via alterations in DNA repair capacity and mitochondrial ultrastructure.
Perez GI, Acton BM, Jurisicova A, Perkins GA, White A, Brown J, Trbovich AM, Kim MR, Fissore R, Xu J, Ahmady A, D’Estaing SG, Li H, Kagawa W, Kurumizaka H, Yokoyama S, Okada H, Mak TW, Ellisman MH, Casper RF, Tilly JL.
Cell Death & Differentiation 14, 524-533. DOI: 10.1038/sj.cdd.4402050
2006
Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein.
Sarai N, Kagawa W, Kinebuchi T, Kagawa A, Tanaka K, Miyagawa K, Ikawa S, Shibata T, Kurumizaka H, Yokoyama S.
Nucleic Acids Research 34, 4429-4437. DOI: 10.1093/nar/gkl562
Role of the N-terminal domain of the human DMC1 protein in octamer formation and DNA binding.
Kinebuchi T, Kagawa W, Kurumizaka H, Yokoyama S.
Journal of Biological Chemistry 280, 28382-28387. DOI: 10.1074/jbc.M503372200
2004
Mutational analyses of the human Rad51-Tyr315 residue, a site for phosphorylation in leukaemia cells.
Takizawa Y, Kinebuchi T, Kagawa W, Yokoyama S, Shibata T, Kurumizaka H.
Genes to Cells 9, 781-790. DOI: 10.1111/j.1365-2443.2004.00772.x
Preferential binding to branched DNA strands and strand-annealing activity of the human Rad51B, Rad51C, Rad51D and Xrcc2 protein complex.
Yokoyama H, Sarai N, Kagawa W, Enomoto R, Shibata T, Kurumizaka H, Yokoyama S.
Nucleic Acids Research 32, 2556-2565. DOI: 10.1093/nar/gkh578
Structural basis for octameric ring formation and DNA interaction of the human homologous-pairing protein Dmc1.
Kinebuchi T, Kagawa W, Enomoto R, Tanaka K, Miyagawa K, Shibata T, Kurumizaka H, Yokoyama S.
Molecular Cell 14, 363-374. DOI: 10.1016/s1097-2765(04)00218-7
2002
Crystal structure of the homologous-pairing domain from the human Rad52 recombinase in the undecameric form.
Kagawa W, Kurumizaka H, Ishitani R, Fukai S, Nureki O, Shibata T, Yokoyama S.
Molecular Cell 10, 359-371. DOI: 10.1016/s1097-2765(02)00587-7
Homologous pairing and ring and filament structure formation activities of the human Xrcc2・Rad51D complex.
Kurumizaka H, Ikawa S, Nakada M, Enomoto R, Kagawa W, Kinebuchi T, Yamazoe M, Yokoyama S, Shibata T.
Journal of Biological Chemistry 277, 14315-14320. DOI: 10.1074/jbc.M105719200
Human Rad54B is a double-stranded DNA-dependent ATPase and has biochemical properties different from its structural homolog in yeast, Tid1/Rdh54.
Tanaka K, Kagawa W, Kinebuchi T, Kurumizaka H, Miyagawa K.
Nucleic Acids Research 30, 1346-1353. DOI: 10.1093/nar/30.6.1346
2001
Homologous pairing promoted by the human Rad52 protein.
Kagawa W, Kurumizaka H, Ikawa S, Yokoyama S, Shibata T.
Journal of Biological Chemistry 276, 35201-35208. DOI: 10.1074/jbc.M104938200
Homologous-pairing activity of the human DNA-repair proteins Xrcc3・Rad51C.
Kurumizaka H, Ikawa S, Nakada M, Eda K, Kagawa W, Takata M, Takeda S, Yokoyama S, Shibata T.
Proceedings of the National Academy of Sciences USA 98, 5538-5543. DOI: 10.1073/pnas.091603098
Human Rad51 amino acid residues required for Rad52 binding.
Kurumizaka H, Aihara H, Kagawa W, Shibata T, Yokoyama S.
Journal of Molecular Biology 291, 537-548. DOI: 10.1006/jmbi.1999.2950
1997
Key determinants of the invasion mechanism of melanoma. Role for a new signaling pathway.
Tímár J, Tóvári J, Szekeres K, Kagawa D, Honn KV.
Advances in Experimental Medicine and Biology 407, 303-310. DOI: 10.1007/978-1-4899-1813-0_45
1996
12-Lipoxygenase in A431 cells: genetic identity, modulation of expression, and intracellular localization.
Hagmann W, Gao X, Timar J, Chen YQ, Strohmaier AR, Fahrenkopf C, Kagawa D, Lee M, Zacharek A, Honn KV.
Experimental Cell Research 228, 197-205. DOI: 10.1006/excr.1996.0317
1993
Activity and protein distribution of 12-lipoxygenase in HEL cells: induction of membrane-association by phorbol ester TPA, modulation of activity by glutathione and 13-HPODE, and Ca(2+)-dependent translocation to membranes.
Hagmann W, Kagawa D, Renaud C, Honn KV.
Prostaglandins 46, 471-477. DOI: 10.1016/0090-6980(93)90066-g